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SCOPE-Seq: a scalable technology for linking live cell imaging and single-cell RNA sequencing
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DOI：10.1186/s13059-018-1607-x.png)
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Abstract
En 中文
Optically decodable beads link the identity of a sample to a measurement through an optical barcode, enabling libraries of biomolecules to be captured on beads in solution and decoded by fluorescence. This approach has been foundational to microarray, sequencing, and flow-based expression profiling technologies. We combine microfluidics with optically decodable beads and show that phenotypic analysis of living cells can be linked to single-cell sequencing. As a proof-of-concept, we demonstrate the accuracy and scalability of our tool called Single Cell Optical Phenotyping and Expression sequencing (SCOPE-Seq) to combine live cell imaging with single-cell RNA sequencing.
Keywords:
Single-cell RNA-Seq
Live cell imaging
Microfluidics
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