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Self-Recognizing L-Glutamine-Conjugated Manganese Oxide Sensors for Salivary Microdroplet Analysis
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DOI：10.1002/anse.202500092.png)
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Abstract
En 中文
Early detection of Alzheimer's disease (AD) remains a critical challenge in neurodiagnostics that necessitates the development of noninvasive, cost-effective, and reliable biosensing platforms. Herein, a self-recognizing L-glutamine-conjugated manganese oxide (Gln-MnO2)-modified electrode is developed for the electrochemical detection of L-glutamine (Gln) in a microdroplet of human saliva, a potential biomarker for early-stage AD. The biofunctionalized MnO2 interface facilitates hydrogen bonding and electrostatic interactions with Gln, eliminating the need for enzymatic recognition elements while enhancing its sensitivity. Electrochemical characterization demonstrates a reversible, adsorption-controlled redox process with an electroactive surface area of 0.225 cm2, high sensitivity (3.16 µA cm−2 nM−1), and a low detection limit (69.7 pM). Real saliva analysis confirms its practical utility, with measured Gln concentrations (6.70 ± 0.93 µM) aligning with reported physiological levels. Furthermore, standard addition experiments yield a recovery of 94.67 ± 5.14%, which also validates its accuracy. The Gln-MnO2 sensor exhibits excellent operational stability over 15 days, with minimal signal loss afterward due to nonspecific adsorption in complex biofluids. The developed platform offers a cost-effective, enzyme-free, and disposable sensing approach for AD biomarker detection, with potential applications in metabolic monitoring and point-of-care diagnostics.
Keywords:
biomarker detection
biomedical diagnostics
electrochemistry
microanalysis
point-of-care testing
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IF:
2.9
Papers:
246
Citations:
393
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