SERS-based multiple biomarker detection using a gold-patterned microarray chip

doi:10.1016/j.molstruc.2012.04.031

SERS-based multiple biomarker detection using a gold-patterned microarray chip

2012-09-01

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Insup Kim

I

Inam-ur-Rehman Junejo

DOI：10.1016/j.molstruc.2012.04.031

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We report a highly sensitive surface-enhanced Raman scattering (SERS)-based immunoassay platform for the multiplex detection of biomarkers. For this purpose, a gold-patterned microarray chip has been fabricated and used as a SERS detection template. Here, a typical sandwich immunocomplex protocol was adopted. Monoclonal antibodies were immobilized on gold patterned substrates, and then antigen solutions and polyclonal antibody-conjugated hollow gold nanospheres (HGNs) were sequentially added for the formation of sandwich immunocomplexes. Antigen biomarkers can be quantitatively assayed by monitoring the intensity change of a characteristic SERS peak of a reporter molecule adsorbed on the surfaces of HGNs. Under optimized assay conditions, the limits of detections (LODs) were determined to be 10 fg/mL for human IgG and 10-100 fg/mL for rabbit IgG. In addition, the SERS-based immunoassay technique can be applied in a wider dynamic concentration range with a good sensitivity compared to ELISA. The proposed method fulfills the current needs of high sensitivity and selectivity which are essential for the clinical diagnosis of a disease. (c) 2012 Elsevier B.V. All rights reserved.

Keywords:

Microarray chip

Hollow gold nanospheres

Surface-enhanced Raman scattering

Multiplex assay

Immunoassay

ELISA

SERS-based multiple biomarker detection using a gold-patterned microarray chip

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