.png){kind=icon}
返回
Single-Cell Transcriptome Analysis Reveals Dynamic Changes in lncRNA Expression during Reprogramming
.png){kind=icon}
OA
AI
DOI：10.1016/j.stem.2014.11.005.png)
.png){kind=icon}
.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
Cellular reprogramming highlights the epigenetic plasticity of the somatic cell state. Long noncoding RNAs (lncRNAs) have emerging roles in epigenetic regulation, but their potential functions in reprogramming cell fate have been largely unexplored. We used single-cell RNA sequencing to characterize the expression patterns of over 16,000 genes, including 437 lncRNAs, during defined stages of reprogramming to pluripotency. Self-organizing maps (SOMs) were used as an intuitive way to structure and interrogate transcriptome data at the single-cell level. Early molecular events during reprogramming involved the activation of Ras signaling pathways, along with hundreds of lncRNAs. Loss-of-function studies showed that activated lncRNAs can repress lineage-specific genes, while lncRNAs activated in multiple reprogramming cell types can regulate metabolic gene expression. Our findings demonstrate that reprogramming cells activate defined sets of functionally relevant lncRNAs and provide a resource to further investigate how dynamic changes in the transcriptome reprogram cell state.
Keyword:
LONG NONCODING RNAS
STEM-CELLS
DNA METHYLATION
CANCER
GENE
STATE
PLURIPOTENCY
MODULATORS
MEMORY
SEQ
AI总结
对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。
期刊
IF:
20.4
论文数:
3.6K
被引数:
3.1W
机构
引用论文
.png){kind=icon}
Natural history and impact on outcomes of acute kidney injury in patients with road traffic injury
PREAI
分享收藏分享收藏分享收藏Inhomogeneous light shift effects on atomic quantum state evolution in non-destructive measurementsOAAI
OAAI分享收藏
查看更多内容