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Single Microbead Floating/Sinking-Enabled Digital Biosensing
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DOI：10.1021/acs.nanolett.6c00238.png)
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Abstract
En 中文
Digital bioassays have emerged as cutting-edge techniques for highly sensitive biomarker analysis. However, the requirement of sophisticated operation and equipment, fully sealed microchambers, and well-trained professionals confines its application to central laboratories. Herein, we present dMSink, a new conceptual mix-and-read digital biosensing platform based on the single microbead (MB) floating/sinking mechanism that is compatible in general biolabs and even in-field testing. Specifically, MBs will float on the surface of a liquid with a finely tuned critical density to ensure that even a single target molecule binding event-introduced extra tiny force (e.g., magnetic force) on the MB is sufficient to induce its sinking. Therefore, only by digitally counting the number of sunken MBs with a conventional microscope can ultrasensitive quantification of diverse biomarkers be facilely achieved. The dMSink empowers the digital bioassay for both speed and sensitivity, significantly simplifying its implementation to in-field multiplexed diagnosis.
Keywords:
Assays
Biomarkers
Cancer
Genetics
Liquids
Digital biosensing
Single microbead
Floating/sinking
Multiplex analysis