.png){kind=icon}
返回
Small-Molecule-Based Lineage Reprogramming Creates Functional Astrocytes
.png){kind=icon}
OA
AI
DOI：10.1016/j.celrep.2016.06.042.png)
.png){kind=icon}
.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
Growing evidence indicates important roles for astrocytes in neurodevelopment and diseases. However, astrocytes and their roles in these processes remain poorly understood. Despite recent progress in reprogramming somatic cells into different types of neural cells, reprogramming to astrocytes has lagged. Here, we show that functional astrocytes can be generated from mammalian fibroblasts using only small molecules. Induced mouse astrocytes resemble primary astrocytes in astrocytic gene expression and epigenomic status and exhibit functional properties in promoting neuronal maturation, glutamate uptake, and calcium signaling. Moreover, these cells can recapitulate the Alexander disease phenotype of protein aggregation when expressing Gfap with a disease-causing mutation. The same compounds can also reprogram human fibroblasts into astroglial progenitor cells that can further mature into functional astrocytes. These chemically induced astrocytes may provide cellular models to uncover roles of astrocytes in normal neurodevelopment and pathogenesis of neurological diseases.
Keyword:
FIBRILLARY ACIDIC PROTEIN
PLURIPOTENT STEM-CELLS
DIRECT CONVERSION
MOUSE FIBROBLASTS
ASTROGLIAL CELLS
NEURONAL CELLS
GENERATION
DISEASE
IDENTIFICATION
EXPRESSION
AI总结
对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。
期刊
IF:
6.9
论文数:
1.7W
被引数:
10.2W
机构
引用论文
Small Molecules Efficiently Reprogram Human Astroglial Cells into Functional Neurons.png)
CELL STEM CELLOAAI
CELL STEM CELLIF20.4
OAAI分享收藏Tgfβ Signal Inhibition Cooperates in the Induction of iPSCs and Replaces Sox2 and cMycCURRENT BIOLOGYOAAI
CURRENT BIOLOGYIF7.5
OAAI分享收藏The Mystery and Magic of Glia: A Perspective on Their Roles in Health and Disease胶质的奥秘和魔力: 对它们在健康和疾病中的作用的看法NEURONOAAI
NEURONIF15
OAAI分享收藏GLUTAMATE TRANSPORTERS IN GLIAL PLASMA-MEMBRANES - HIGHLY DIFFERENTIATED LOCALIZATIONS REVEALED BY QUANTITATIVE ULTRASTRUCTURAL IMMUNOCYTOCHEMISTRYNEURONOAAI
NEURONIF15
OAAI分享收藏
查看更多内容