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Snapshot Projection Optical Tomography
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DOI：10.1103/PhysRevApplied.13.054048.png)
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We present a plenoptic microscopy configuration for three-dimensional (3D) snapshot imaging, which is dual telecentric and can directly record true projection images corresponding to different viewing angles. It also allows the blocking of high-angle stray rays without sacrificing the light collection efficiency. This configuration, which we call snapshot projection optical tomography (SPOT), arranges an objective lens and a microlens array (MLA) in a 4F telecentric configuration (where F denotes the focal length of the lens) and places an aperture stop at the back focal plane of a relay lens. We develop a forward imaging model for SPOT, which can also be applied to existing light-field microscopy techniques using a MLA as a tube lens. Using the developed system, we demonstrate snapshot 3D imaging of various fluorescent beads and a biological cell, which confirms the capability of SPOT for imaging specimens with an extended fluorophore distribution as well as isolated fluorochromes. The transverse and vertical resolutions are measured to be 0.8 mu m and 1.6 mu m, respectively.
Keyword:
LIGHT-FIELD
MICROSCOPY
RECONSTRUCTION
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4.4
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被引数:
2.8W
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