.png){kind=icon}
返回
SSSMuG: Same Sample Sequential Multi-Glycomics
.png){kind=icon}
PRE
AI
DOI：10.1021/acs.analchem.3c04928.png)
.png){kind=icon}
原文求助.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
The mammalian glycome is structurally complex and diverse, composed of many glycan classes such as N- and O-linked glycans, glycosaminoglycans (GAGs), glycosphingolipids (GSLs), and other distinct glycan features such as polysialic acids (PolySia), sulfation, and proteoglycan attachment stubs. Various methods are used to analyze these different components of the glycome, but they require prefractionated/partitioned samples to target each glycan class individually. To address this need for a knowledge of the relationship between the different glycan components of a biological system, we developed a sequential release workflow for analysis of multiple conjugated glycan classes (PolySia, GAGs, GSL glycans, N-glycans, and O-glycans) from the same tissue lysate, termed SSSMuG & horbar;Same Sample Sequential Multi-Glycomics. With this sequential glycan release approach, five glycan classes were characterized (or four glycan classes plus proteomics) using enzymatic or chemical release from a single sample immobilized on a polyvinylidene difluoride membrane. The various released glycan classes were then analyzed by HPLC and MS techniques using commonly available analytical setups. Compared to single glycan class release approaches, SSSMuG was able to identify more glycans and more proteins with higher-intensity analytical peaks and provide a better comparative normalization of the different glycan classes of the complex glycome. To this end, the SSSMuG technology workflow will be a foundation for a paradigm shift in the field, transforming glycoanalytics and facilitating the push toward multiglycomics and systems glycobiology.
Keyword:
PROTEIN EXTRACTION
POLYSIALIC ACID
TISSUE
SULFATE
期刊
IF:
6.7
论文数:
4.7W
被引数:
15.9W
机构
引用论文
Total cellular glycomics allows characterizing cells and streamlining the discovery process for cellular biomarkers总细胞糖组学允许表征细胞并简化细胞生物标志物的发现过程
OAAI
OAAI分享收藏Characterization of Glycosaminoglycan Disaccharide Composition in Astrocyte Primary Cultures and the Cortex of Neonatal Rats星形胶质细胞原代培养物和新生大鼠皮层中糖胺聚糖二糖组成的表征
OAAI
OAAI分享收藏Quantitative analysis of chondroitin sulfate disaccharides from human and rodent fixed brain tissue by electrospray ionization-tandem mass spectrometry.png)
GLYCOBIOLOGYOAAI
GLYCOBIOLOGYIF3.3
OAAI分享收藏Serial in-solution digestion protocol for mass spectrometry-based glycomics and proteomics analysisMOLECULAR OMICSOAAI
MOLECULAR OMICSIF2.4
OAAI分享收藏
查看更多内容