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Super-Resolution solid-state NMR Spectroscopy
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DOI：10.1007/s10858-026-00490-5.png)
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Abstract
En 中文
Solid-state NMR spectroscopy is often limited by low spectral resolution, a problem typically addressed using fast magic-angle spinning (MAS) and H-1 detection, which require costly specialized hardware. Here, we demonstrate that the super-resolution method-previously applied in solution-state NMR-can be successfully implemented in solid-state NMR to enhance resolution. Applying dynamic number of scans (DNS) sampling to 2D C-13-C-13 DARR experiments on the AP205 capsid protein yielded an effective doubling of resolution, halving peak widths from similar to 180 Hz to similar to 87 Hz. Furthermore, DNS acquisition provides a significant advantage over post-acquisition apodization of conventional data with a 20% gain in sensitivity, yielding 309 more detectable peaks with 20% more sequential contacts and 25% more long-range contacts. This method is simple to implement and provides a powerful, accessible strategy to greatly improve the quality of solid-state NMR spectra applicable at all MAS frequencies.
Keywords:
2-DIMENSIONAL NMR
PROTEIN-STRUCTURE
SENSITIVITY
RESOLUTION
ENHANCEMENT
ASSIGNMENT
SPECTRA
Journal
J
IF:
1.9
Papers:
18
Citations:
0