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Synergistic integration of bidirectional PER and DNAzyme cascades with ZIF-8 quenching for detection of aflatoxin B1
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DOI：10.1039/D6AY00837B.png)
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Abstract
En 中文
Aflatoxin B1 (AFB1); classified as a Group 1 human carcinogen by the International Agency for Research on Cancer (IARC); poses significant risks to food safety and public health owing to its potent hepatotoxicity and genotoxicity; as well as its remarkable thermal and chemical stability. Conventional detection methods face limitations such as complex instrumentation; high operational costs; and the risk of cross-reactivity. Herein; we developed a highly sensitive fluorescent biosensor leveraging the synergistic integration of bidirectional primer exchange reaction (BPER) amplification; DNAzyme-mediated cleavage; and ZIF-8-induced fluorescence quenching. The sensor employs an AFB1-triggered cascade reaction wherein target recognition induces reassembly of split DNAzyme fragments into a catalytically active enzyme. This enzyme cleaves a FAM-labeled reporter probe; generating a truncated product with diminished affinity for ZIF-8 nanoparticles. This results in a fluorescence “turn-on” signal quantitatively correlated with AFB1 concentration. The system achieves high sensitivity and specificity by combining programmable DNA length modulation with ZIF-8's efficient quenching properties; offering a robust platform for rapid; on-site AFB1 detection in food samples.
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