.png){kind=icon}
.png){kind=icon}
Return
Systemic metabolic rebalance and regulatory network reconstruction drive high-yield production of sucrose isomerase by nutrient deficient Bacillus amyloliquefaciens
.png){kind=icon}
PRE
AI
M
Y
L
R
A
Y
路
DOI：10.1016/j.cej.2026.180416.png)
.png){kind=icon}
Original request for help.png){kind=icon}
Share.png){kind=icon}
Save
Abstract
En 中文
• Food-grade B. amyloliquefaciens chassis was built via D-alanine auxotrophy. • Weakening marker alr1 ensured 100% plasmid stability and higher copy number. • Co-expressing ald restored intracellular L-Ala/D-Ala metabolic homeostasis. • Mutating cre motif in PamyS alleviated CCR and boosted transcription 2.9-fold. • Sucrose isomerase titer reached 1527.23 U/mL in a 200-L bioreactor.
Keywords:
Bacillus amyloliquefaciens
Food-grade expression
Elements optimization
Metabolic engineering
Sucrose isomerase
Isomaltulose
Journal
IF:
13.2
Papers:
7.2W
Citations:
48.5W
Organization
Cited Papers