The Vibrio cholerae chitin utilization program

doi:10.1073/pnas.0308707101

The Vibrio cholerae chitin utilization program

2004-02-17

504

OA

AI

K

Karin Lederballe Meibom

L

Li, XBB

A

Alex Toftgaard Nielsen

DOI：10.1073/pnas.0308707101

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Chitin, an insoluble polymer of GIcNAc, is an abundant source of carbon, nitrogen, and energy for marine microorganisms. Microarray expression profiling and mutational studies of Vibrio cholerae growing on a natural chitin surface, or with the soluble chitin oligosaccharides (GIcNAc)(2-6), GIcNAc, or the glucosamine dimer (GIcN)(2) identified three sets of differentially regulated genes. We show that (i) ChiS, a sensor histidine kinase, regulates expression of the (GIcNAc)(2-6) gene set, including a (GIcNAc)(2) catabolic operon, two extracellular chitinases, a chitoporin, and a PiIA-containing type IV pilus, designated ChiRP (chitin-regulated pilus) that confers a significant growth advantage to V. cholerae on a chitin surface; (ii) GIcNAc causes the coordinate expression of genes involved with chitin chemotaxis and adherence and with the transport and assimilation of GIcNAc; (iii) (GIcN)(2) induces genes required for the transport and catabolism of nonacetylated chitin residues; and (iv) the constitutively expressed MSHA pilus facilitates adhesion to the chitin surface independent of surface chemistry. Collectively, these results provide a global portrait of a complex, multistage V. cholerae program for the efficient utilization of chitin.

Keyword:

MANNOSE-SENSITIVE HEMAGGLUTININ

MOLECULAR-CLONING

INFECTIOUS-DISEASE

CATABOLIC CASCADE

GLOBAL CLIMATE

EL-TOR

BACTERIUM

FURNISSII

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The Vibrio cholerae chitin utilization program

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