Tutorial: interrogating biology using organoid technologies

Jeroen M. Bugter

doi:10.1038/s41596-026-01447-6

2026-09-24

PRE

Jeroen M. Bugter *

Simon T. Schafer *

Roland Rad *

DOI：10.1038/s41596-026-01447-6

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类器官为研究基础生物学提供了多功能平台，补充了传统细胞培养和体内模型。其独特特征，如自组织和精确操纵的可能性，已推动在组织稳态、发育和疾病方面的关键发现。本教程概述了广泛应用于从研究细胞命运决定、细胞-细胞或细胞-微生物相互作用到遗传疾病、物种进化和转化/临床问题的类器官技术和方案。通过聚焦于成体干细胞衍生的肠类器官和人 pluripotent stem cell 衍生的脑类器官，我们突出了具有不同特征的模型，这些特征共同反映了类器官系统、组织类型和研究背景的更广泛范围。对于这些模型不支持的应用，我们讨论了来自其他组织的类器官。我们还概述了该领域的当前挑战，并突出了有望塑造未来方向的新兴创新。本教程概述了类器官技术在从研究细胞命运决定和细胞-细胞相互作用到研究遗传疾病、物种进化和临床问题的广泛应用中的使用。

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Nature Protocols

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