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Using deep learning to quantify neuronal activation from single-cell and spatial transcriptomic data
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DOI：10.1038/s41467-023-44503-5.png)
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Abstract
En 中文
Neuronal activity-dependent transcription directs molecular processes that regulate synaptic plasticity, brain circuit development, behavioral adaptation, and long-term memory. Single cell RNA-sequencing technologies (scRNAseq) are rapidly developing and allow for the interrogation of activity-dependent transcription at cellular resolution. Here, we present NEUROeSTIMator, a deep learning model that integrates transcriptomic signals to estimate neuronal activation in a way that we demonstrate is associated with Patch-seq electrophysiological features and that is robust against differences in species, cell type, and brain region. We demonstrate this method's ability to accurately detect neuronal activity in previously published studies of single cell activity-induced gene expression. Further, we applied our model in a spatial transcriptomic study to identify unique patterns of learning-induced activity across different brain regions in male mice. Altogether, our findings establish NEUROeSTIMator as a powerful and broadly applicable tool for measuring neuronal activation, whether as a critical covariate or a primary readout of interest. Neuronal activity is associated with transcriptional changes. Here, the authors present a deep learning model that integrates single cell transcriptomic signals to estimate neuronal activation.
Keywords:
ANTERIOR-CHAMBER
LIVER
HEPATOCYTES
DYNAMICS
BIOLOGY
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