.png){kind=icon}
返回
Y chromosomal STR analysis using Pyrosequencing technology
.png){kind=icon}
PRE
AI
DOI：10.1016/j.fsigen.2008.11.010.png)
.png){kind=icon}
原文求助.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
Analysis of Y chromosome STIR markers has proven to be useful in forensic cases where the samples contain a mixture of DNA from several individuals. STR markers are commonly genotyped based on length separation of PCR products. In this study we evaluated if Pyrosequencing can be used as an alternative method for determining Y-STR variants. In total 70 unrelated Swedish males were typed for the Y chromosomal markers (DYS19, DYS389 I-II, DYS390, DYS391, DYS392, DYS393 and DYS438) using Pyrosequencing. Using the 8 markers, 57 unique haplotypes were observed with a discrimination capacity of 0.81. At four loci, the Pyrosequencing analysis revealed sequence variants. The sequence variants were found in the DYS389 11, DYS390, DYS391, and DYS393 loci in frequencies between 1.43% and 14.3%. Pyrosequencing has here been shown to be a useful tool for typing Y chromosomal STRs and the method can provide a complement to conventional forensic Y STIR analyses. Moreover, the Pyrosequencing method can be used to rapidly evaluate novel markers. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
Keyword:
Pyrosequencing
Y chromosome STR
Forensic identification
AI总结
对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。
期刊
IF:
3.1
论文数:
2.8K
被引数:
5.8K
机构
引用论文
.png){kind=icon}
查看更多内容