.png){kind=icon}
返回
Z-ring Structure and Constriction Dynamics in E-coil
.png){kind=icon}
OA
AI
DOI：10.3389/fmicb.2017.01670.png)
.png){kind=icon}
.png){kind=icon}
分享.png){kind=icon}
收藏
摘要
En 中文
The Z-ring plays a central role in bacterial division. It consists of FtsZ filaments, but the way these reorganize in the ring-like structure during septation remains largely unknown. Here, we measure the effective constriction dynamics of the ring. Using an oscillating optical trap, we can switch individual rod-shaped E. coil cells between horizontal and vertical orientations. In the vertical orientation, the fluorescent Z-ring image appears as a symmetric circular structure that renders itself to quantitative analysis. In the horizontal orientation, we use phase-contrast imaging to determine the extent of the cell constriction and obtain the effective time of division. We find evidence that the Z-ring constricts at a faster rate than the cell envelope such that its radial width (inwards from the cytoplasmic membrane) grows during septation. In this respect, our results differ from those recently obtained using photoactivated localization microscopy (PALM) where the radial width of the Z-ring was found to be approximately constant as the ring constricts. A possible reason for the different behavior of the constricting Z-rings could be the significant difference in the corresponding cell growth rates.
Keyword:
bacterial division
Z-ring
optical tweezers
fluorescence microscopy
sub-pixel measurements
AI总结
对已上传原文的论文进行重点信息的提取，主要内容包括：简要概述、研究摘要、背景介绍、关键亮点、图文解析、展望与总结。
期刊
IF:
4.5
论文数:
4.1W
被引数:
16.6W
机构
引用论文
.png){kind=icon}
A fast and scalable fault injection framework to evaluate multi/many-core soft error reliabilityOAAI
OAAI分享收藏
分享收藏
查看更多内容