3D+t Morphological Processing: Applications to Embryogenesis Image Analysis

Miguel Luengo-Oroz

doi:10.1109/TIP.2012.2197007

3D+t Morphological Processing: Applications to Embryogenesis Image Analysis

2012-08-01

14

OA

AI

M

Miguel Luengo-Oroz *

D

David Pastor-Escuredo

C

Carlos Castro‐González

José L. Rubio-Guivernau

L

Louise Duloquin

M

María J. Ledesma‐Carbayo

DOI：10.1109/TIP.2012.2197007

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Abstract

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We propose to directly process 3D + t image sequences with mathematical morphology operators, using a new classification of the 3D + t structuring elements. Several methods (filtering, tracking, segmentation) dedicated to the analysis of 3D + t datasets of zebrafish embryogenesis are introduced and validated through a synthetic dataset. Then, we illustrate the application of these methods to the analysis of datasets of zebrafish early development acquired with various microscopy techniques. This processing paradigm produces spatio-temporal coherent results as it benefits from the intrinsic redundancy of the temporal dimension, and minimizes the needs for human intervention in semi-automatic algorithms.

Keywords:

3D+t mathematical morphology

in-vivo light microscopy

spatio-temporal structuring elements

zebrafish embryogenesis

3D+t Morphological Processing: Applications to Embryogenesis Image Analysis

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