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3DCellComposer − A versatile pipeline utilizing 2D cell segmentation methods for 3D cell segmentation
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DOI：10.1016/j.ymeth.2025.07.007.png)
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Abstract
En 中文
• Developed an approach to use 2D cell segmenters to produce 3D segmentations. • Previously developed metrics for evaluating 2D segmentations without human input. • Extended metrics to 3D, enabling optimization of segmentation parameters. • Obtained results for 3D multichannel images better than existing 3D methods.
Keywords:
Cell segmentation
Nuclear segmentation
Spatial proteomics
Multiplexed fluorescence imaging
Imaging mass spectroscopy
3D microscopy
Tissue images