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A biophysicist’s guide to translocation of double-stranded nucleic acids through solid-state nanopores
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DOI：10.1016/j.plrev.2026.03.002.png)
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Abstract
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• Practical guide to optimise signal-to-noise ratio, translocation time and throughput in solid-state nanopore experiments. • Systematic framework linking voltage, pore geometry and buffer conditions to experimental performance. • Physical principles underlying translocation and parameter-dependent behaviour, including electrophoresis, electro-osmotic flow, hydrodynamic friction and entropic effects. • Concepts transferable to broader nanoscale transport and sensing systems.
Keywords:
Solid-state Nanopores
Single-molecule biophysics
Double-stranded nucleic acids
Polymer translocation
Electrophoresis
Electro-osmotic flow
Experimental optimisation
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