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A CRISPR/Cas12a−MXene Nanozyme Platform for Universal Detection of Trace DNA
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PRE
AI
S
Y
M
张
Z
Q
唐
DOI：10.1021/acssensors.5c03702.png)
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Abstract
En 中文
The instrument-free detection of specific DNA sequences is critical for point-of-care diagnostics yet remains challenging. To address this, we developed a universal colorimetric biosensing platform integrating the CRISPR/Cas12a system with MXene nanozymes. Target recognition by the Cas12a/crRNA complex triggers trans-cleavage of single-stranded DNA inhibitors, restoring the peroxidase-like activity of MXenes to produce a visible signal. The platform achieved a detection limit of 132 copies/μL for the methicillin-resistant Staphylococcus aureus mecA gene. By incorporating an exponential circular DNA (CirDNA) amplification strategy and rationally designed crRNAs for single-base discrimination, sensitivity was further enhanced, enabling detection of the B-raf proto-oncogene V600E mutation down to 109 aM with 0.5% variant allele frequency. The platform also showed excellent reproducibility and high recovery rates in simulated clinical samples. This work provides a low-cost, label/instrument-free, and highly sensitive universal approach through the synergistic combination of CRISPR/Cas12a programmability, MXene nanozyme activity, and CirDNA amplification.
Keywords:
Absorption
Colorimetry
Fluorescence
Genetics
Two dimensional materials
DNA detection
CRISPR/Cas12a
MXenes
antibiotic-resistant bacteria
circulating tumor DNA
Journal
IF:
9.1
Papers:
976
Citations:
2.6W