.png){kind=icon}
Return
A Digital Microfluidic Approach to Proteomic Sample Processing
.png){kind=icon}
OA
AI
DOI：10.1021/ac900522a.png)
.png){kind=icon}
.png){kind=icon}
Share.png){kind=icon}
Save
Abstract
En 中文
A common characteristic for proteomic analyses is the need for extensive biochemical processing. Digital microfluidics (DMF), a technique characterized by the manipulation of discrete microdroplets; (100 nL-10 mu L) on an open array of electrodes, is a good match for carrying out rapid, automated solution-phase reactions. Here, we report a DMF-based method integrating several common processing steps in proteomics, including reduction, alkylation, and enzymatic digestion. Fluorogenic assays were used to quantitatively evaluate the kinetics and reproducibility of each reaction step, and MALDI-MS was used for qualitative confirmation. The method is fast, facile, and reproducible, and thus has the potential to be a useful new tool in proteomics.
Keywords:
ON-A-CHIP
PROTEIN DIGESTION
SOL-GEL
REACTOR
DIAGNOSTICS
SEPARATION
ACTUATION
TRYPSIN
AI Summary
Key information extracted from the uploaded paper, including a brief overview, abstract, background, key highlights, visual analysis, and future outlook.
Journal
IF:
6.7
Papers:
4.7W
Citations:
15.9W
Organization
Cited Papers
An integrated digital microfluidic lab-on-a-chip for clinical diagnostics on human physiological fluids.png)
LAB ON A CHIP
LAB ON A CHIPIF5.4
PREAI
ShareSaveShareSaveShareSaveShareSave
ShareSaveEnzymatic microreactor-on-a-chip:: Protein mapping using trypsin immobilized on porous polymer monoliths molded in channels of microfluidic devicesANALYTICAL CHEMISTRY
ANALYTICAL CHEMISTRYIF6.7
PREAI
ShareSave
View more