.png){kind=icon}
.png){kind=icon}
Return
A digital microfluidic interface between solid-phase microextraction and liquid chromatography-mass spectrometry
.png){kind=icon}
PRE
AI
DOI：10.1016/j.chroma.2016.03.029.png)
.png){kind=icon}
Original request for help.png){kind=icon}
Share.png){kind=icon}
Save
Abstract
En 中文
We introduce a method to couple solid-phase microextraction (SPME) with HPLC-MS using digital microfluidics (DMF). In the new system, SPME fibers are used to extract analytes from complex sample solutions, after which the analytes are desorbed into solvent droplets in a DMF device. The open geometry of DMF allows straightforward insertion of SPME fibers without requiring a complicated interface, and automated droplet manipulation enables multiplexed processing of the fibers. In contrast to other multiplexed SPME elution interfaces, the low volumes inherent to DMF allow for pre-concentration of analytes prior to analysis. The new SPME-DMF-HPLC-MS method was applied to the quantification of pg/mL-level free steroid hormones in urine. We propose that this new method will be useful for a wide range of applications requiring cleanup and pre-concentration with convenient coupling to high-performance analytical techniques. (C) 2016 Elsevier B.V. All rights reserved.
Keywords:
Digital microfluidics
Solid-phase microextraction
HPLC-MS
SPME
Urine
Steroid
Hormone
AI Summary
Key information extracted from the uploaded paper, including a brief overview, abstract, background, key highlights, visual analysis, and future outlook.
Journal
IF:
4
Papers:
3.2W
Citations:
5.0W