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A facile electrochemical immunosensor based on EDTA-Pb2+complexation reaction
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DOI：10.1016/j.talanta.2024.125957.png)
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Abstract
En 中文
The sensitivity of electrochemical (EC) sensors has been improved through the development of multiple approaches. However, the majority of EC sensors were limited in their practical application by high costs or tedious procedures. Herein, based on ethylenediaminetetraacetic acid (EDTA)-Pb 2+ complexation reaction, a facile and affordable immunosensor was designed. Pb 2+ -magnesium silicate hydrate was served as the sensing substrate. The immunorecognition process was carried out in the Eppendorf tube, and antibody-functionalized Pb 2+ -pol- ydopamine was utilized as immunoprobe. In the tube, the quantitative and appropriate excess of EDTA was introduced to complex with Pb 2+ on the immunoprobes. The remaining EDTA was added to the sensing substrate surface to coordinate with some Pb 2+ in it. This leaded to the reduction of the EC signal of Pb 2+ , which was related to the antigen concentration. Using prostate-specific antigen as the model analyte, the sensitive detection was realized with a low limit of detection (30.49 fg mL -1 ). Remarkably, the assay results were available within 24 min, sensibly faster than the most existing EC sensors.
Keywords:
Polydopamine
Magnesium silicate hydrate
Prostate-specific antigen
Electrochemical sensor