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A flow-batch luminometer
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DOI：10.1016/j.microc.2012.10.009.png)
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Abstract
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The traditional flow chemiluminescence (CL) systems typically use flow cells in spiral shape mounted in front of a photomultiplier tube (PMT). The sample is transported by using carrier fluids, undergoes dispersion, and mixes with reagents and CL reaction occurs before reaching the flow cell. As a consequence, the maximum of the chemiluminescence intensity is not exploited and the sensitivity is significantly diminished. In this work, these drawbacks were overcome by using a flow-batch approach and a large area silicon photodiode instead of PMT in order to build a simple and automatic luminometer for CL measurements. The feasibility of the proposed flow-batch luminometer was demonstrated in the determination of vitamin B-12 in injection ampoules, yielding limits of detection and quantification (0.11 and 0.36 mu g L-1, respectively), recovery rates (between 97.8 and 102.1%), relative standard deviations (RSD<2.2%, n=3) and sample throughput (about 72 h(-1)). Thus, it was possible to both project and build a simple, flexible, versatile and automatic luminometer, while keeping the excellent characteristics of the previous flow-batch analyzer such as: low reagent and sample consumption and minimal waste generation. (C) 2012 Elsevier B.V. All rights reserved.
Keywords:
Flow-batch luminometer
Chemiluminescence
Injection ampoules
Vitamin B-12
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