.png){kind=icon}
Return
A multi-kingdom genetic barcoding system for precise clone isolation
.png){kind=icon}
OA
AI
DOI：10.1038/s41587-025-02649-1.png)
.png){kind=icon}
Original request for help.png){kind=icon}
Share.png){kind=icon}
Save
Abstract
En 中文
Cell-tagging strategies with DNA barcodes have enabled the analysis of clone size dynamics and clone-restricted transcriptomic landscapes in heterogeneous populations. However, isolating a target clone that displays a specific phenotype from a complex population remains challenging. Here we present a multi-kingdom genetic barcoding system, CloneSelect, which enables a target cell clone to be triggered to express a reporter gene for isolation through barcode-specific CRISPR base editing. In CloneSelect, cells are first stably tagged with DNA barcodes and propagated so that their subpopulation can be subjected to a given experiment. A clone that shows a phenotype or genotype of interest at a given time can then be isolated from the initial or subsequent cell pools stored during the experiment using CRISPR base editing. CloneSelect is scalable and compatible with single-cell RNA sequencing. We demonstrate the versatility of CloneSelect in human embryonic kidney 293T cells, mouse embryonic stem cells, human pluripotent stem cells, yeast cells and bacterial cells.
Keywords:
EPIGENETIC MEMORY
LINEAGE
CELLS
DIFFERENTIATION
DNA
ADAPTATION
INDUCTION
DYNAMICS
DESIGN
FATE
Journal
IF:
41.7
Papers:
1.2W
Citations:
10.1W
Organization
Cited Papers
High-resolution lineage tracking reveals travelling wave of adaptation in laboratory yeast.png)
Nature
OAAI
NatureIF0
OAAIShareSaveShareSaveShareSaveSimultaneous single-cell profiling of lineages and cell types in the vertebrate brainNATURE BIOTECHNOLOGYOAAI
NATURE BIOTECHNOLOGYIF41.7
OAAIShareSaveStudying clonal dynamics in response to cancer therapy using high-complexity barcodingNATURE MEDICINE
NATURE MEDICINEIF50
PREAI
ShareSaveUncoupling of sgRNAs from their associated barcodes during PCR amplification of combinatorial CRISPR screensPLOS ONEOAAI
PLOS ONEIF0
OAAIShareSaveEpigenetic Variation between Human Induced Pluripotent Stem Cell Lines Is an Indicator of Differentiation CapacityCELL STEM CELLOAAI
CELL STEM CELLIF20.4
OAAIShareSave
View more