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A nanozyme-enzyme hybrid electrochemical platform based on CeO2-MoS2/Au for selective lactate monitoring in human sweat
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DOI：10.1016/j.ijoes.2026.101315.png)
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Abstract
En 中文
This study reports a nanozyme-enhanced electrochemical platform for quantifying lactate in exercise-induced sweat, constructed by modifying a glassy carbon electrode with a CeO2-MoS2 composite decorated with Au nanoparticles and immobilized lactate oxidase. Structural characterization confirmed crystalline CeO2 (8 nm) with mixed Ce3 +/Ce4+ states, layered MoS2 (0.62 nm spacing), and uniformly distributed AuNPs, forming a conductive, high-surface-area catalytic interface. The composite significantly reduced the H2O2reduction over-potential, enabling sensor operation at 0.0 V vs Ag/AgCl and minimizing interference from coexisting sweat metabolites. Electrochemical impedance spectroscopy showed a reduction in charge transfer resistance from-580 Omega (bare electrode) to-220 Omega after nanozyme modification, supporting accelerated electron transport. The sensor exhibited rapid amperometric response (<10 s), a broad linear range of 0.05-20 mM, and a sensitivity of 25.0 A & sdot;mM(-1) & sdot;cm(-2), with a detection limit of 0.02 mM, fully encompassing physiological sweat lactate levels from rest to intense exercise. High selectivity was demonstrated, with responses to 5 mM lactate remaining within 97-103 % in the presence of 0.2 mM ascorbate, 0.2 mM urate, and 5 mM glucose, while D-lactate produced < 2 % of the L-lactate signal. The device showed excellent reproducibility (RSD 4.7 %), repeatability (RSD 3.2 %), and long-term stability, retaining-90 % sensitivity after four weeks of storage at 4 degrees C. Recovery tests in artificial sweat yielded 96-97 %, and real-sample analysis agreed within 4 % of a reference colorimetric assay. These results establish a laboratory-validated nanozyme-enzyme catalytic interface on a rigid glassy carbon electrode and provide a reproducible foundation for subsequent integration and validation on flexible, epidermal-relevant platforms for wearable sweat monitoring.
Keywords:
Peroxidase mimicry
Cerium oxide nanozyme
Lactate oxidase
Glassy carbon electrode
Amperometric sweat analysis
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