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A programmable Raman plate reader based on a modified 3D printer for high-throughput screening
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DOI：10.1117/12.3081319.png)
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Abstract
En 中文
In this paper, we extend the characterization of RamanBot, an automated Raman screening platform constructed by repurposing a Cartesian-based commercial 3D printer. The system features a redesigned extrusion head that integrates all excitation and collection optics, enabling programmable, high-throughput screening of diverse microplate formats using standard G-code. We characterized the platform's performance on a 384-well microplate, demonstrating high signal-to-noise ratios (SNR) with acquisition times as short as 2 seconds and negligible optical cross-interference. The system's analytical versatility was validated through two complementary studies. First, a quantitative analysis of glycerol concentrations yielded a highly linear response (R-2 > 0.99) and a Limit of Detection (LOD) of 6.9 mM. Second, we evaluated the system's qualitative classification robustness by discriminating between structurally similar purine bases (Adenine and Guanine) within a high-interference background of L-Tyrosine and L-Tryptophan. Despite severe spectral overlap that reduced discriminative variance to less than 2%, a linear Support Vector Machine (SVM) classifier achieved 96.1% accuracy on independent test samples. These results establish the proposed platform as a robust, low-cost solution for both sensitive quantitative sensing and complex biological identification tasks.
Keywords:
Raman Spectroscopy
high-throughput screening
Microplate reader
Modified 3D printer
Support vector machine
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