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A split fluorescent reporter with rapid and reversible complementation
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DOI：10.1038/s41467-019-10855-0.png)
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Abstract
En 中文
Interactions between proteins play an essential role in metabolic and signaling pathways, cellular processes and organismal systems. We report the development of splitFAST, a fluorescence complementation system for the visualization of transient protein-protein interactions in living cells. Engineered from the fluorogenic reporter FAST (Fluorescence-Activating and absorption-Shifting Tag), which specifically and reversibly binds fluorogenic hydroxybenzylidene rhodanine (HBR) analogs, splitFAST displays rapid and reversible complementation, allowing the real-time visualization of both the formation and the dissociation of a protein assembly.
Keywords:
PROTEIN INTERACTIONS
MAP KINASE
VISUALIZATION
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