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A temperature-triggered phase separation system for stabilizing bioactive protein structure and function
DOI:10.1016/j.talanta.2026.129675.png)
Abstract
En 中文
• Temperature-triggered reversible water-in-water protein protection strategy. • Tunable cloud point and pH matching various proteins. • Outer phase provides air-liquid isolation and minimizes interface-induced aggregation. • Inner phase stabilizes protein conformation via reducing molecular collisions.
Keywords:
protein stabilization
phase separation
temperature-triggered
protein conformation
water-in-water system

