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A user-friendly two-color super-resolution localization microscope

delete2015-01-23
delete34
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OA
AI
T
Teng Zhao
王颖 cover
王颖 (Ying Wang)
Y
Yuanliang Zhai
X
Xiaoxuan Qu
A
Aifang Cheng
S
Shengwang Du
M
M. M. T. Loy *
DOI：10.1364/OE.23.001879delete
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Abstract

Abstract

En 中文
We report a robust two-color method for super-resolution localization microscopy. Two-dye combination of Alexa647 and Alexa750 in an imaging buffer containing COT and using TCEP as switching regent provides matched and balanced switching characteristics for both dyes, allowing simultaneous capture of both on a single camera. Active sample locking stabilizes sample with 1nm accuracy during imaging. With over 4,000 photons emitted from both dyes, two-color superresolution images with high-quality were obtained in a wide range of samples including cell cultures, tissue sections and yeast cells. (C) 2015 Optical Society of America
Keywords:
FLUORESCENT-PROBES
FLUOROPHORES
NANOSCOPY
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Journal

Optics Express cover
Optics Express
IF:
3.3
Papers:
6.1W
Citations:
14.3W

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No organization information available