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A visible dual-applicable imidazole-Schiff base fluorescent probe for precise determination of copper ions in actual aqueous solutions and living cells
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DOI：10.1016/j.molstruc.2026.146095.png)
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Abstract
En 中文
A novel imidazole-based hydrazide fluorescent probe, (E)-N '-((1H-imidazol-2-yl)methylene)- 4-(4,5-diphenyl-1H-imidazol-2-yl)benzohydrazide (HDB), was designed and synthesized. The chemical structure of HDB was fully characterized by FT-IR, H-1 NMR, C-13 NMR, and HR-MS, confirming the successful synthesis of the target product. Subsequently, the sensing performance of HDB was systematically investigated via fluorescence spectroscopy and ultraviolet-visible absorption spectroscopy. The probe HDB exhibited naked-eye distinguishable recognition of Cu2+ with high sensitivity and selectivity in the EtOH/H2O (1:1, v/v), where fluorescence quenching was triggered by the chelation between HDB and Cu2+. Performance evaluation results demonstrated that the detection limit of HDB for Cu2+ was as low as 0.38 nM. Job's plot analysis revealed a 1:1 binding stoichiometry between HDB and Cu2+, with a binding constant of 1.765x10(5) M-1. Cyclic experiments involving the alternate addition of Cu2+ and Na(2)EDTA verified the excellent reusability of HDB. The practical applicability of HDB was validated through quantitative detection of Cu2+ in real water samples, where the obtained recoveries fell within an acceptable range, indicating its feasibility for trace Cu2+ analysis in environmental water matrices. Furthermore, this study explored the potential application of HDB in fingerprint detection, providing a new avenue for the innovation of fingerprint identification and anti-counterfeiting technologies. Notably, HDB achieved visualized monitoring of Cu2+ in living cells, which further expands its application prospects in the biomedical field.
Keywords:
Imidazole
Acylhydrazone
Copper (II)
Fluorescent probe
Biological imaging
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4.7
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3.5W
Citations:
6.6W
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