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An alternative framework for fluorescence correlation spectroscopy
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DOI：10.1038/s41467-019-11574-2.png)
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Abstract
En 中文
Fluorescence correlation spectroscopy (FCS), is a widely used tool routinely exploited for in vivo and in vitro applications. While FCS provides estimates of dynamical quantities, such as diffusion coefficients, it demands high signal to noise ratios and long time traces, typically in the minute range. In principle, the same information can be extracted from microseconds to seconds long time traces; however, an appropriate analysis method is missing. To overcome these limitations, we adapt novel tools inspired by Bayesian non-parametrics, which starts from the direct analysis of the observed photon counts. With this approach, we are able to analyze time traces, which are too short to be analyzed by existing methods, including FCS. Our new analysis extends the capability of single molecule fluorescence confocal microscopy approaches to probe processes several orders of magnitude faster and permits a reduction of photo-toxic effects on living samples induced by long periods of light exposure.
Keywords:
HIGH COUNT-RATE
SINGLE-MOLECULE
BAYESIAN-INFERENCE
IN-VITRO
MODEL SELECTION
INFINITE HMMS
TIME-SERIES
DYNAMICS
FCS
STATES
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