.png){kind=icon}
.png){kind=icon}
Return
Ancestral Protein-Based Lighting
.png){kind=icon}
OA
AI
DOI：10.1002/adma.202420303.png)
.png){kind=icon}
.png){kind=icon}
Share.png){kind=icon}
Save
Abstract
En 中文
Protein-optoelectronics is a paradigm toward eco-designed and sustainable technologies. The challenge is, however, how to preserve the native activity of proteins upon device fabrication/operation in non-native environments (solvents, organic/inorganic interfaces, and working temperatures/irradiations). Herein, a new vision to identify and design ancestral-like fluorescent proteins (FPs) is proposed. Using ancestral sequence reconstruction (ASR) out of a large dataset (221) of the best modern FPs suitable for photon down-conversion in bio-hybrid light-emitting diodes (Bio-HLEDs) a historical-genetic reconstruction (family tree) was obtained, identifying a possible common ancestral FP. This computationally designed protein is produced in bacteria, featuring outstanding photoluminescence quantum yields in solution (e.g., 90%/80% for green-/red-emitting forms) and a strong tendency to agglomerate in polymer coatings. This resulted in red-emitting Bio-HLEDs that outperformed the reference with ≈2-fold enhanced stabilities. The resplendent green-/red-emission of ancestral-like FP itself and its respective devices led us to coin this new protein as QuetzalFP. Overall, it is set in ASR as an effective concept to reshape protein-optoelectronics allowing us to identify i) many interesting ancestral FPs for lighting and ii) QuetzalFP as stepping-stone platform for protein engineering.
Keywords:
ancestral sequence reconstruction
bio-hybrid light-emitting diodes
fluorescent protein
protein-lighting
photon manipulation
AI Summary
Key information extracted from the uploaded paper, including a brief overview, abstract, background, key highlights, visual analysis, and future outlook.
Journal
IF:
26.8
Papers:
3.4W
Citations:
46.0W