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Anti-inflammatory and non-toxic effects of Doyerea emetocathartica aqueous extract in mice
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DOI：10.1016/j.jep.2025.119534.png)
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Abstract
En 中文
Ethnopharmacological relevance: Doyerea emetocathartica is a Cucurbitaceae known as cabe & ccedil;a de negro and used by North-eastern Brazil and South-eastern Mexico communities to treat rheumatism, inflammation and pain. Despite ethnopharmacological reports, there is currently a lack of evidence about its chemical composition, toxicity and pharmacological effects. Aim of the study: This study aimed to investigate the chemical composition of the aqueous extract of D. emetocathartica stems (DEAQ), evaluate its acute oral toxicity, mutagenicity and its anti-inflammatory profile in mice. Material and methods: The DEAQ chemical composition was evaluated through HPLC-MS/MS. Acute oral toxicity was assessed for 14 days after the oral administration of DEAQ in a single dose of 2000 mg kg- 1. The mutagenic assessment was carried out through micronucleus test in mice. The DEAQ anti-inflammatory activity was evaluated at doses of 25, 50 and 100 mg kg- 1 throught models of carrageenan-induced paw edema, peritonitis and air pouch. Results: Using HPLC-MS/MS the cayaponoside D2 was identified as the most abundant compound in DEAQ. Oral DEAQ administration showed no signs of toxicity or mutagenicity. All tested DEAQ doses had anti-edematogenic effects, decreased the migration of total leukocytes and neutrophils and decreased the levels of TNF-alpha and IL-1 beta. Conclusions: The in vivo tests results indicated that DEAQ showed strong anti-inflammatory activity, with possible cayaponoside D2 involvement, highlighting the absence of toxicity and genotoxicity for the dose and route used. Furthermore, the study provides chemical and pharmacological evidences about the therapeutic use of D. emetocathartica in folk medicine.
Keywords:
Cabe & ccedil
a de negro''
Mass spectrometry
Saponins
Acute toxicity
Anti-inflammatory activity
Cytokines
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