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Aptamer-based CRISPR-Cas12a fluorescent biosensors for serum biomarkers detection
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DOI：10.1039/D6AN00123H.png)
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Abstract
En 中文
The CRISPR-Cas12a system enables sensitive nucleic acid detection due to its programmability; trans-cleavage activity; and biocompatibility. To expand its applications beyond nucleic acid analysis; aptamers have emerged as an ideal recognition element owing to their high specificity; design flexibility; ease of modification and low cost. The integration of Cas12a with aptamers enables the conversion of target-binding signals into nucleic acid recognition signals; thereby combining molecular recognition with signal amplification for the analysis of non-nucleic acid targets. This review begins with a concise overview of the Cas12a system's working mechanism and features; with particular emphasis on recent advances in Cas12a-aptamerbased fluorescent biosensors for serum biomarker detection. It also discusses their advantages and limitations; current challenges; and future prospects.
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3.3
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1.6W
Citations:
3.3W
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