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Aqueous two-phase system formed by polyethylene glycol methyl ether 550 and choline-based ionic liquids: from phase diagrams to application in the extraction of proteins
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Tairan Eutimio dos SantosM
Milena Santos LeiteR
Ranyere Lucena de SouzaM
Matheus Mendonça PereiraM
Mara G. FreireC
Cleide Mara Faria SoaresA
A.Álvaro Silva Lima* DOI：10.1016/j.bej.2026.110225.png)
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Abstract
En 中文
• ATPS composed of PEGME 550 and cholinium-based ionic liquids can be formed • Phase formation is primarily driven by intermolecular interactions • Optimal BSA (K=0.81) and IgG (K=0.70) partitioning was achieved in [Ch]Cl-based ATPS • Molecular docking simulations confirmed the experimental partitioning trends • FTIR spectroscopy confirmed the structural integrity of IgG after separation
Keywords:
ATPS
cholinium-based ionic liquids
protein extraction
phase formation
molecular docking
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3.8
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6.2K
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