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Assembling of recombinant calmodulin with modulating function on solid surface

delete2003-09-01
delete3
PRE
AI
E
Eiry Kobatake *
S
Shigeya Suzuki
M
Masuo Aizawa
DOI：10.1016/S0925-4005(03)00324-1delete
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Abstract

Abstract

En 中文
Many types of proteins express their functions with conformation change in various biosystems. For the construction of biosensing system consisting of such functional proteins, the development of techniques for assembling of proteins with retaining their functions even on a solid substrate is required. Calmodulin was selected as a model protein for molecular assembly. Calmodulin expresses modulating function by conformation change with calcium binding. In the present study, some cysteine residues were introduced at the N-terminus of calmodulin by genetic engineering, and the resulting engineered calmodulin could be assembled efficiently with oriented manner. The modulating function of calmodulin on a solid-phase surface was also demonstrated. (C) 2003 Elsevier B.V. All rights reserved.
Keywords:
calmodulin
self-assembling
cysteine
coordinated function
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Key information extracted from the uploaded paper, including a brief overview, abstract, background, key highlights, visual analysis, and future outlook.

Journal

Sensors and Actuators B-Chemical cover
Sensors and Actuators B-Chemical
IF:
7.7
Papers:
3.3W
Citations:
12.6W

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No organization information available