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Bacterial cellulose–Morchella sextelata mycelium composite disks as scalable, bioactive scaffolds: A promising platform for cultured meat
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DOI：10.1016/j.carbpol.2026.125700.png)
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Abstract
En 中文
Cultured meat, as an emerging method for obtaining protein, is receiving widespread attention. Developing biocompatible, edible scaffolding materials is a critical bottleneck for the industrialization of cultured meat. In this study, we developed a series of microbia-derived, biomass-based composite disk carriers by integrating bacterial cellulose (BC) with mycelium (Myc) from Morchella sextelata via a facile and minimally-processed blend-casting method. This composite is a potential, low-cost alternative to traditional BC–mushroom chitosan (BCMC) carriers in our previous work. Results indicated that mycelial integration enriched the surface with nitrogen-containing functional groups and optimized wettability to a range of 68°–76°. The resulting micro-nano topology provided robust mechanical interlocking, which correlated with reduced cell sheet detachment compared to BCMC benchmarks. Biological assays demonstrated that BC–Myc disks significantly accelerated the proliferation of C2C12 and DF-1 cells, achieving near 100% confluence within 5 days. Immunofluorescence revealed that the carriers supported mature C2C12 myogenic differentiation with continuous, multi-nucleated fibers and promoted DF-1 adipogenic transdifferentiation with high fatty acid synthase expression. Overall, BC–Myc disks outperformed BCMC in supporting tissue maturation and interfacial stability. This work establishes a scalable, low-cost, and circular economy strategy for fabricating scaffolding biomaterials, providing a promising proof-of-concept platform for cultured meat production.
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