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Best practice for CRISPR design using current tools and resources

delete2019-07-01
delete8
PRE
AI
M
Mark Thomas
D
David Parry-Smith
V
Vivek Iyer *
DOI:10.1016/j.ymeth.2019.05.019delete
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Abstract

Abstract

En 中文
Users facing the task of designing gRNAs for a CRISPR-mutagenesis experiment are typically confronted with a large variety of possible tools and existing libraries. Here we examine the design principles for such resources, and suggest a best practice which allows a user to evaluate and effectively use any of the existing CRISPR design tools or genome-wide libraries.
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Methods cover
Methods
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4.3
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4.8K
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wellcome trust sanger institute
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Citations: 17