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BID-seq: The Quantitative and Base-Resolution Sequencing Method for RNA Pseudouridine
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DOI：10.1021/acschembio.2c00881.png)
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Abstract
En 中文
Quantitative and base-resolution sequencing methods are critical to investigations of the biological functions of diverse RNA modifications. These methods may also be employed for clinical studies and clinical applications in the future. In this In Focus article, we introduce and discuss the development of Bisulfite-Induced Deletion sequencing (BID-seq) for quantitatively detecting mRNA pseudouridine (W) modifications at base resolution.
Keywords:
MESSENGER-RNA
GENE-EXPRESSION
5-METHYLCYTOSINE
METHYLOME