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Binding buffer optimization for TEOS-modified magnetite nanoparticle-based DNA extraction method
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DOI：10.1016/j.matchemphys.2025.130622.png)
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Abstract
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Magnetic nanoparticle-based DNA extraction is a well-known method. In its application, TEOS is the most common component used as a coating agent. TEOS-modified magnetic nanoparticle (TMNP) was prepared using a ratio of 0.5 ml TEOS/g MNP. The resulting TMNP has a crystalline size of 19.8 nm and has ferromagnetic properties. Selection of the appropriate binding buffer with TMNP based on the response of DNA concentration, purity, and integrity was GuSCN at a concentration of 2.5 M and dissolved at pH 6.5. This combination of TMNP and binding buffer can extract DNA of various types of bacteria and produce DNA in the range of 2.55-12.45 mu g and DNA purity at 260/280 is 1.58-2.27. the type of bacteria that can be isolated is Escherichia coli, Pseudomonas aeruginosa, Salmonella sp., Salmonella paratyphi, Bacillus subtilis, Staphylococcus aureus, Enterobacter aerogenes, Shigella sonnei, Staphylococcus epidermidis, and Mycobacterium tuberculosis. The extracted DNA can also be used for probe-based and intercalating dye-based qPCR.
Keywords:
Binding buffer
DNA extraction
Magnetite
TEOS
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