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Biogeographic Variation and Functional Pathways of the Gut Microbiota in Celiac Disease
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DOI：10.1053/j.gastro.2022.06.088.png)
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Abstract
En 中文
BACKGROUND & AIMS: Genes and gluten are necessary but insufficient to cause celiac disease (CeD). Altered gut micro -biota has been implicated as an additional risk factor. Vari-ability in sampling site may confound interpretation and mechanistic insight, as CeD primarily affects the small intestine. Thus, we characterized CeD microbiota along the duodenum and in feces and verified functional impact in gnotobiotic mice.METHODS: We used 16S rRNA gene sequencing (Illumina) and predicted gene function (PICRUSt2) in duodenal biopsies (D1, D2 and D3), aspirates, and stool from patients with active CeD and controls. CeD alleles were determined in consented par-ticipants. A subset of duodenal samples stratified according to similar CeD risk genotypes (controls DQ2-/- or DQ2 thorn /- and CeD DQ2 thorn /-) were used for further analysis and to colonize germ -free mice for gluten metabolism studies.RESULTS: Micro -biota composition and predicted function in CeD was largely determined by intestinal location. In the duodenum, but not stool, there was higher abundance of Escherichia coli (D1), Prevotella salivae (D2), and Neisseria (D3) in CeD vs controls. Predicted bacterial protease and peptidase genes were altered in CeD and impaired gluten degradation was detected only in mice colonized with CeD microbiota.CONCLUSIONS: Our re-sults showed luminal and mucosal microbial niches along the gut in CeD. We identified novel microbial proteolytic pathways involved in gluten detoxification that are impaired in CeD but not in controls carrying DQ2, suggesting an association with active duodenal inflammation. Sampling site should be considered a confounding factor in microbiome studies in CeD.
Keywords:
Celiac Disease
Gut Microbiota
Duodenum
Pro-teases
Gnotobiotic Mice
Journal
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