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Breaking the cold chain: solutions for room temperature preservation of mosquitoes leading to high quality reference genomes
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DOI：10.1093/gigascience/giag061.png)
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Abstract
En 中文
The Earth BioGenome Project (EBP) is a global endeavour to produce reference genomes for all described eukaryotic species. The majority of described species are arthropods, which tend to be small and require taxonomic expertise to identify to species level. Therefore, the ability to collect and preserve specimens in a suitable way for long read and Hi-C data generation using very simple approaches with minimal infrastructure is certain to be important in scaling up reference genome generation. Field collections requiring dry ice or dry shippers can be logistically challenging to arrange, are notoriously expensive, and DNA degrades rapidly if ultra-cold temperature is not maintained, which is devastating given how expensive and time consuming field work can be.
Keywords:
Arthropods
Reference genomes
Field collection
Room temperature preservation
Hi-C data