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Bridging the sensitivity gap in amplification-free CRISPR diagnostics: An engineering-driven redesign
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DOI：10.1016/j.trac.2026.119043.png)
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Abstract
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• Five key engineering renovation directions for the diagnostic performance enhancement of amplification-free CRISPR. • In-depth analysis of the structure and cleaving mechanism for precise renovation. • Summarizing the shortcomings of the current amplification-free CRISPR approach and the future development directions.
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