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Ca2+ effects on manganese oxidation by the bacterial multicopper oxidase complex Mnx
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DOI：10.1016/j.jinorgbio.2026.113396.png)
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Abstract
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• Mn oxidation by bacterial multicopper oxidase Mnx in the presence of Ca2+ is studied. • Ca modestly affects the first step of the reaction, Mn(II)-Mn(III) oxidation. • Ca enhances the second step, disproportionation of Mnx-bound dimeric Mn(III). • The acceleration is cooperative in nature, revealing that two Ca2+ act together. • One Ca binds for every 2 Mn in the proposed Mnx's initial product, a MnO2 tetramer.
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