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Candida glycerinogenes-Promoted ?-Pinene and Squalene Co- production Strategy Based on ?-Pinene Stress
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DOI：10.1021/acs.jafc.3c00199.png)
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Abstract
En 中文
alpha-Pinene is a naturally occurring monoterpene, which is widely used in fragrances, cosmetics, and foods. Due to the high cellular toxicity of alpha-pinene, this work considered the application of Candida glycerinogenes, an effective industrial strain with high resistance, in alpha-pinene synthesis. It was found that alpha-pinene-induced stress resulted in an intracellular accumulation of reactive oxygen species with an increased formation of squalene as a cytoprotective compound. As squalene is a downstream product in the mevalonate (MVA) pathway for alpha-pinene synthesis, a strategy based on the promotion of alpha-pinene and squalene co-production under alpha-pinene stress is proposed. By introducing the alpha-pinene synthesis pathway and enhancing the MVA pathway, the production of both alpha-pinene and squalene is increased. We have demonstrated that intracellular synthesis of alpha-pinene is effective in promoting squalene synthesis. The generation of intercellular reactive oxygen that accompanies alpha-pinene synthesis promotes squalene synthesis with a resultant cellular protection and upregulation of MVA pathway genes that facilitate alpha-pinene production. In addition, we have overexpressed phosphatase and introduced NPP as a substrate to synthesize alpha-pinene, where co-dependent fermentation yielded 208 mg/L squalene and 12.8 mg/L alpha-pinene. This work establishes a viable strategy to promote terpene-co-dependent fermentation based on stress.
Keywords:
Candida glycerinogenes
?-pinene
ROS
MVA pathway
squalene
Journal
IF:
6.2
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4.5W
Citations:
15.3W
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Cited Papers