.png){kind=icon}
Return
CEL-Seq: Single-Cell RNA-Seq by Multiplexed Linear Amplification
.png){kind=icon}
OA
AI
DOI：10.1016/j.celrep.2012.08.003.png)
.png){kind=icon}
.png){kind=icon}
Share.png){kind=icon}
Save
Abstract
En 中文
High-throughput sequencing has allowed for unprecedented detail in gene expression analyses, yet its efficient application to single cells is challenged by the small starting amounts of RNA. We have developed CEL-Seq, a method for overcoming this limitation by barcoding and pooling samples before linearly amplifying mRNA with the use of one round of in vitro transcription. We show that CEL-Seq gives more reproducible, linear, and sensitive results than a PCR-based amplification method. We demonstrate the power of this method by studying early C. elegans embryonic development at single-cell resolution. Differential distribution of transcripts between sister cells is seen as early as the two-cell stage embryo, and zygotic expression in the somatic cell lineages is enriched for transcription factors. The robust transcriptome quantifications enabled by CEL-Seq will be useful for transcriptomic analyses of complex tissues containing populations of diverse cell types.
Keywords:
GENE-EXPRESSION
BLASTOMERE
AI Summary
Key information extracted from the uploaded paper, including a brief overview, abstract, background, key highlights, visual analysis, and future outlook.
Journal
IF:
6.9
Papers:
1.7W
Citations:
10.2W
Organization
Cited Papers
Characterization of the single-cell transcriptional landscape by highly multiplex RNA-seq.png)
GENOME RESEARCHOAAI
GENOME RESEARCHIF5.5
OAAIShareSaveShareSaveRestriction of mesendoderm to a single blastomere by the combined action of SKN-1 and a GSK-3β homolog is mediated by MED-1 and-2 in C-elegansMOLECULAR CELLOAAI
MOLECULAR CELLIF16.6
OAAIShareSaveQuantitative analysis of mRNA amplification by in vitro transcriptionNUCLEIC ACIDS RESEARCHOAAI
NUCLEIC ACIDS RESEARCHIF13.1
OAAIShareSave
View more