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Cerium oxide nanoparticles decrease inflammation in J774 cells
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DOI：10.1096/fasebj.22.1_supplement.758.2.png)
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Abstract
En 中文
The use of nanomaterials in biological systems has gained attention due to the unique properties that these materials possess. Cerium oxide (CeO2) is a rare earth oxide that, on the nanomolar scale, has been reported to exhibit antioxidant properties. Cerium oxide acts as a regenerative free radical scavenger due to its valence and oxygen defects. In this study, we investigated the ability of CeO2 nanoparticles to reduce reactive oxygen species (ROS) and inflammatory mediator production in J774 murine macrophage cells in vitro. J774 cells were pretreated with various concentrations of CeO2 nanoparticles 24 hr prior to LPS/IFN‐γ stimulation. Our results showed that J774 cells internalized CeO2 particles as assessed by electron microscopy and atomic force microscopy. The cerium particles localized around the mitochondria, a major producer of ROS in the cell. Treatment with CeO2 at concentrations under 1 uM caused no toxicity. Cerium oxide pretreated cells, when stimulated with LPS/IFN‐γ, showed decreased oxidative stress. Furthermore, CeO2 pretreatment decreased inflammatory cytokines IL‐6 and TNF‐α in LPS/IFN‐γ stimulated cells. Taken together, these studies suggest that cerium oxide nanoparticles are effective in reducing ROS and inflammatory mediator production, and may constitute a novel therapeutic tool for the treatment of inflammation.
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