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Characterizing Nanoparticle Size and Composition using Microfluidic Raman Diffusion-Ordered Spectroscopy
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DOI：10.1039/D5AY01250C.png)
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Abstract
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We show that microfluidic Raman Diffusion-Ordered SpectroscopY (Raman-DOSY) can be used to simultaneously characterize the size and the chemical composition of nanoparticles in solution. In these experiments; we inject a solution of nanoparticles into one half of a microfluidic channel; and solvent into the other. After the injection; the nanoparticles diffuse into the solvent-filled part of the channel at a rate determined by their diffusion coefficient. By analyzing the time-dependent Raman spectrum in the initially solvent-filled part of the channel; we determine the size (from the diffusion coefficient) and characterize the chemical composition (from the corresponding Raman spectra) of the nanoparticles. Within about 1 hour we obtain both the size and chemical-structure information of polystyrene beads with diameters of 20; 50; and 100 nm. We further demonstrate that this method can distinguish nanoparticles of varying sizes in mixed samples. These results show that microfluidic Raman-DOSY is a promising method for combined size- and composition analysis of nanoparticles.
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