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Clinical outcome of an SCNT-derived MSTN knockout buffalo: a case study
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DOI：10.3389/fgeed.2026.1822169.png)
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Abstract
En 中文
Myostatin (MSTN) gene knockout has attracted considerable interest for enhancing meat production in livestock species due to its well-established role as a negative regulator of skeletal muscle growth. In this study; we report the clinical outcome of a bi-allelic MSTN knockout buffalo produced via somatic cell nuclear transfer (SCNT) that failed to survive beyond 100 days of age. The MSTN knockout calf exhibited a double-muscling phenotype; which was validated by changes in mRNA expression of transcripts associated with increased muscle mass and reduced subcutaneous fat. In addition; the calf had a telomere length similar to that of age-matched calves produced through artificial insemination. However; serum biochemistry analysis revealed decreased levels of globulin; GGTP; ALP; and bilirubin; alongside increased concentrations of AST; urea; blood urea nitrogen; uric acid; phosphorus; and potassium. Cytokine profiling (including IFN-γ; IL-1α; IL-1β; IL-4; IL-6; IL-8; IL-10; IL-17α; IL-36Rα; TNF-α; MIP-1α; MIP-1β; MCP-1; IP-10; and VEGF-α) demonstrated dysregulation of several inflammatory and chemotactic mediators. This case report highlights potential health and welfare challenges associated with producing MSTN knockout buffalo through cloning-based approaches.
Keywords:
buffalo
genome editing
somatic cell nuclear transfer
immunometabolic regulation
muscle growth
myostatin knockout
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Cited Papers